Development of 111In-labeled liposomes for vulnerable atherosclerotic plaque imaging.
Identifieur interne : 000219 ( Main/Exploration ); précédent : 000218; suivant : 000220Development of 111In-labeled liposomes for vulnerable atherosclerotic plaque imaging.
Auteurs : RBID : pubmed:24337605English descriptors
- KwdEn :
- Animals, Apolipoproteins E (genetics), Humans, Indium Radioisotopes (chemistry), Lipids (chemistry), Liposomes (chemistry), Macrophages (metabolism), Male, Mice, Mice, Knockout, Nitrilotriacetic Acid (chemistry), Observer Variation, Phosphatidylcholines (chemistry), Phosphatidylserines (chemistry), Plaque, Atherosclerotic (radionuclide imaging), Rabbits, Time Factors, Tomography, Emission-Computed, Single-Photon, Tomography, X-Ray Computed.
- MESH :
- chemical , chemistry : Indium Radioisotopes, Lipids, Liposomes, Nitrilotriacetic Acid, Phosphatidylcholines, Phosphatidylserines.
- chemical , genetics : Apolipoproteins E.
- metabolism : Macrophages.
- radionuclide imaging : Plaque, Atherosclerotic.
- Animals, Humans, Male, Mice, Mice, Knockout, Observer Variation, Rabbits, Time Factors, Tomography, Emission-Computed, Single-Photon, Tomography, X-Ray Computed.
Abstract
Macrophage infiltration is a common characteristic feature of atherosclerotic-vulnerable plaques. Macrophages recognize phosphatidylserine (PS) exposed on the surface of apoptotic cells, which triggers the engulfment of the apoptotic cells by macrophages through phagocytosis. In this study, we prepared radiolabeled PS liposomes for detection of vulnerable plaques.
DOI: 10.2967/jnumed.113.123158
PubMed: 24337605
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of 111In-labeled liposomes for vulnerable atherosclerotic plaque imaging.</title><author><name sortKey="Ogawa, Mikako" uniqKey="Ogawa M">Mikako Ogawa</name><affiliation wicri:level="1"><nlm:affiliation>Medical Photonics Research Center, Hamamatsu University School of Medicine, Hamamatsu, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Medical Photonics Research Center, Hamamatsu University School of Medicine, Hamamatsu</wicri:regionArea></affiliation></author><author><name sortKey="Umeda, Izumi O" uniqKey="Umeda I">Izumi O Umeda</name></author><author><name sortKey="Kosugi, Mutsumi" uniqKey="Kosugi M">Mutsumi Kosugi</name></author><author><name sortKey="Kawai, Ayumi" uniqKey="Kawai A">Ayumi Kawai</name></author><author><name sortKey="Hamaya, Yuka" uniqKey="Hamaya Y">Yuka Hamaya</name></author><author><name sortKey="Takashima, Misato" uniqKey="Takashima M">Misato Takashima</name></author><author><name sortKey="Yin, Hongxia" uniqKey="Yin H">Hongxia Yin</name></author><author><name sortKey="Kudoh, Takayuki" uniqKey="Kudoh T">Takayuki Kudoh</name></author><author><name sortKey="Seno, Masaharu" uniqKey="Seno M">Masaharu Seno</name></author><author><name sortKey="Magata, Yasuhiro" uniqKey="Magata Y">Yasuhiro Magata</name></author></titleStmt><publicationStmt><date when="2014">2014</date><idno type="RBID">pubmed:24337605</idno><idno type="pmid">24337605</idno><idno type="doi">10.2967/jnumed.113.123158</idno><idno type="wicri:Area/Main/Corpus">000253</idno><idno type="wicri:Area/Main/Curation">000253</idno><idno type="wicri:Area/Main/Exploration">000219</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Apolipoproteins E (genetics)</term><term>Humans</term><term>Indium Radioisotopes (chemistry)</term><term>Lipids (chemistry)</term><term>Liposomes (chemistry)</term><term>Macrophages (metabolism)</term><term>Male</term><term>Mice</term><term>Mice, Knockout</term><term>Nitrilotriacetic Acid (chemistry)</term><term>Observer Variation</term><term>Phosphatidylcholines (chemistry)</term><term>Phosphatidylserines (chemistry)</term><term>Plaque, Atherosclerotic (radionuclide imaging)</term><term>Rabbits</term><term>Time Factors</term><term>Tomography, Emission-Computed, Single-Photon</term><term>Tomography, X-Ray Computed</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Indium Radioisotopes</term><term>Lipids</term><term>Liposomes</term><term>Nitrilotriacetic Acid</term><term>Phosphatidylcholines</term><term>Phosphatidylserines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Apolipoproteins E</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Macrophages</term></keywords><keywords scheme="MESH" qualifier="radionuclide imaging" xml:lang="en"><term>Plaque, Atherosclerotic</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Male</term><term>Mice</term><term>Mice, Knockout</term><term>Observer Variation</term><term>Rabbits</term><term>Time Factors</term><term>Tomography, Emission-Computed, Single-Photon</term><term>Tomography, X-Ray Computed</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Macrophage infiltration is a common characteristic feature of atherosclerotic-vulnerable plaques. Macrophages recognize phosphatidylserine (PS) exposed on the surface of apoptotic cells, which triggers the engulfment of the apoptotic cells by macrophages through phagocytosis. In this study, we prepared radiolabeled PS liposomes for detection of vulnerable plaques.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">24337605</PMID><DateCreated><Year>2014</Year><Month>01</Month><Day>03</Day></DateCreated><DateCompleted><Year>2014</Year><Month>03</Month><Day>03</Day></DateCompleted><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1535-5667</ISSN><JournalIssue CitedMedium="Internet"><Volume>55</Volume><Issue>1</Issue><PubDate><Year>2014</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Journal of nuclear medicine : official publication, Society of Nuclear Medicine</Title><ISOAbbreviation>J. Nucl. Med.</ISOAbbreviation></Journal><ArticleTitle>Development of 111In-labeled liposomes for vulnerable atherosclerotic plaque imaging.</ArticleTitle><Pagination><MedlinePgn>115-20</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.2967/jnumed.113.123158</ELocationID><Abstract><AbstractText Label="UNLABELLED">Macrophage infiltration is a common characteristic feature of atherosclerotic-vulnerable plaques. Macrophages recognize phosphatidylserine (PS) exposed on the surface of apoptotic cells, which triggers the engulfment of the apoptotic cells by macrophages through phagocytosis. In this study, we prepared radiolabeled PS liposomes for detection of vulnerable plaques.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">PS liposomes were prepared by lipid film hydration. Phosphatidylcholine (PC) liposomes were prepared as controls. Liposomes (100 or 200 nm) were generated by an extruder to produce PS100, PS200, PC100, and PC200 liposomes. These were then radiolabeled by encapsulating (111)In-nitrilotriacetic acid using an active-loading method. (111)In liposomes were incubated with cultured macrophages for 2 h, and the uptake level was measured. For biodistribution studies, the (111)In liposomes were injected intravenously into ddY mice. In addition, the (111)In liposomes were injected into apolipoprotein E-deficient (apoE-/-) mice, and the aortas were harvested for autoradiography and oil red O staining. For SPECT imaging, (111)In liposomes were injected intravenously into Watanabe heritable hyperlipidemic rabbits and scanned 48 h after injection.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The radiochemical yields were greater than 95% for all the prepared (111)In liposomes. The level of in vitro uptake by macrophages was 60.5, 14.7, 32.0, and 14.4 percentage injected dose per milligram of protein for (111)In-PS100, (111)In-PC100, (111)In-PS200, and (111)In-PC200, respectively. In biodistribution studies, high spleen uptake was seen with PC liposomes. Liver uptake was high for all liposomes but was lowest with (111)In-PS200. The blood half-lives were 3.2, 22.0, 3.6, and 7.4 min for (111)In-PS100, (111)In-PC100, (111)In-PS200, and (111)In-PC200, respectively. The distribution of (111)In-labeled PS liposomes into atherosclerotic regions determined by autoradiography was well matched with the results of oil red O staining in apoE-/- mice. The target-to-nontarget ratios were 2.62, 2.23, 3.27, and 2.51 for (111)In-PS100, (111)In-PC100, (111)In-PS200, and (111)In-PC200, respectively. The aorta was successfully visualized by SPECT at 48 h after (111)In-labeled PS liposome injection; however, high liver uptake was also observed.</AbstractText><AbstractText Label="DISCUSSION" NlmCategory="CONCLUSIONS">From the in vitro uptake study, it has been demonstrated that macrophage targeting was accomplished by PS modification. Also, an atherosclerotic region was successfully detected by (111)In-PS200 in apoE-/- mice and Watanabe heritable hyperlipidemic rabbits in vivo. Liposome modification to obtain slower blood clearance and lower liver uptake would be required to improve the SPECT images.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ogawa</LastName><ForeName>Mikako</ForeName><Initials>M</Initials><Affiliation>Medical Photonics Research Center, Hamamatsu University School of Medicine, Hamamatsu, Japan.</Affiliation></Author><Author ValidYN="Y"><LastName>Umeda</LastName><ForeName>Izumi O</ForeName><Initials>IO</Initials></Author><Author ValidYN="Y"><LastName>Kosugi</LastName><ForeName>Mutsumi</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Kawai</LastName><ForeName>Ayumi</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Hamaya</LastName><ForeName>Yuka</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Takashima</LastName><ForeName>Misato</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Yin</LastName><ForeName>Hongxia</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Kudoh</LastName><ForeName>Takayuki</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Seno</LastName><ForeName>Masaharu</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Magata</LastName><ForeName>Yasuhiro</ForeName><Initials>Y</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>12</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Nucl Med</MedlineTA><NlmUniqueID>0217410</NlmUniqueID><ISSNLinking>0161-5505</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Apolipoproteins E</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Indium Radioisotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Lipids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Liposomes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Phosphatidylcholines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Phosphatidylserines</NameOfSubstance></Chemical><Chemical><RegistryNumber>KA90006V9D</RegistryNumber><NameOfSubstance>Nitrilotriacetic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Apolipoproteins E</DescriptorName><QualifierName MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indium Radioisotopes</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Lipids</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Liposomes</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Macrophages</DescriptorName><QualifierName MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mice, Knockout</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Nitrilotriacetic Acid</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Observer Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Phosphatidylcholines</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Phosphatidylserines</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Plaque, Atherosclerotic</DescriptorName><QualifierName MajorTopicYN="Y">radionuclide imaging</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Rabbits</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tomography, Emission-Computed, Single-Photon</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tomography, X-Ray Computed</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">SPECT</Keyword><Keyword MajorTopicYN="N">atherosclerosis</Keyword><Keyword MajorTopicYN="N">liposome</Keyword><Keyword MajorTopicYN="N">phosphatidylserine</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="aheadofprint"><Year>2013</Year><Month>12</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>12</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>12</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>3</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24337605</ArticleId><ArticleId IdType="pii">jnumed.113.123158</ArticleId><ArticleId IdType="doi">10.2967/jnumed.113.123158</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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